My Lab Notebook

Sea lice bismark alignments: mox script here: https://gannet.fish.washington.edu/metacarcinus/mox_jobs/20190814_BmrkCalig.sh data here: https://gannet.fish.washington.edu/metacarcinus/Salmo_Calig/analyses/20190814_Calig/ bismark summary report shows limited mehtylation as discussed on slack here. This isn’t that surprising as the global DNA methylation ELISA data showed Female 1 only 0.56% methylated and Female 2 3.38% methylated. We had tried WGBS with high-ish... [Read More]

Respirometry Ran 1 respirometry trial with 1 animal/vial; each animal from different silo filling vials (tried something different): filled 2.5qt HD buckets halfway with 20um filtered treatment water (this would probably be better with a vaccuum filtration unit, syringe filtering is pretty terrible) placed 12 vials in 24-well plate (as... [Read More]

Experiment Set-up labeled silos Tote #2 = 2.1-2.12 back to front and right to left Tote #3 = 3.1-3.12 back to front and right to left)](https://drive.google.com/open?id= [Read More]

Adding 1.5 year old juveniles to experiment [Read More]

Sequim Dig Kaitlyn and I took the 5:35am edmonds-kingston ferry to meet Liz and Steven at the Natural Resources Lab in Sequim Bay by 7:15am [Read More]