Strip-spawned juveniles (March 17, 2019) from ambient or varibable low pH exposed broodstock
-
Installed new two-channel pump head and skinnier tubing (norprene 14) for feeding
- did algae counts on cellometer
- inflow:
- Low pH header: 1.41 x 10^6 cells/mL
- Ambient header: 1.24 x 10^6 cells/mL
- outflow:
- H1T8: 1 x 10^5 cells/mL
- Last H2 tray (empty): 8 x 10^4 cells/mL
- rate = 10ml/3 sec. = 200mL/min. = 12L/hour = 288L/day
- 1 x 10^5 cells/mL * 288,000mL = 2.8 x 10^10 cells/day
- counting with the sedgewick slide I only got 5 x 10^3 cells/mL (~5 cells/square * 1000 mm^3 squares = 5000 cells/mL)
- 1.44 * 10^9 cells/day conclusions:
- They are seeing 2,880 M cells /day counted by cellometer and 144 M cells/day counted by sedgewick
- They should be getting 22.4 M cells/day.
- By cellometer, they are getting 2880M/22.4 ~128X more than needed and by sedgewick they are getting 144M/22.4M = ~6X more
- They are still getting more than needed, but are now getting less than before so it’s likely better.
- inflow:
- shuffled trays around because cascading water system gives top trays more food
before shuffling
H2 stack (top tray is “Elev. Par. Hist. from T3 Elevated” but i had moved the label down to the second to bottom tray - where I was going to rotate that tray)
H2_T5 (Elevated parents)
H2_T7 (Ambient parents)
H2_T3 (Elevated parents)
H2_T1 (Ambient parents)
H2_T2 (Ambient parents, conical 2 ~2000 animals)
H2_T6 (Elevated parents; extras)
Animals from H2_T6 (Elevated parents; extras)
after shuffling
order (top to bottom) H2_T2 H2_T6 H2_T1 H2_T7 H2_T5 H2_T3