Thur. Dec. 19, Geoduck Brood Conditioning

Broodstock Experimental Setup

Matt set up a new algae feeing system!

all parts are listed below under the heading “Equipment received”
This is an image of the tank empty
This is an image of the tank with algae being pumped in. The algae flows in by the float valve and is then pumped straight up through the flow meter. Excess algae (not going through the flow meter) gets overflowed back into the tank
The next two images show where the algae goes after it passes the flow meter, up and over into the manifold which feeds the totes

There were a total of ~120 broodstock that came in on 12/11/19 from Port Gamble. And there were 16 that came in on 10/17/19 (not sure where these came from; these will be used for commercial spawning)
Matt and I split out the broodstock randomly into the 4 experimental tanks:
- B1 (pH7.2)
- B2 (amb)
- B4 (amb)
- B5 (pH7.2)
- we added 23 healthy looking animals/tank
Remaining 12/11/19 animals (~20) were put back in tank B6 to be used in commercial spawning

B1:

B2:

B4:

B5:

B6:

B3 (silos with juveniles from Fall exp):

Annual report year 2 (due end of month)
- for my github repos Pt. Whitney OA experiments and Geoduck genome paper:
  - create repo releases and forward URLs to Steven
  - update readme.txts
- add image and video links to SAFSOA website
Manuscript directions
- Brood reproduction:
  - finish developing VTG assay
  - finish histology analysis
  - don’t worry about Ctenidia, Gonad, or Siphon samples for now
  - focus on brood; not the egg, sperm, or fertilized eggs
- Transgenerational effect of variable pH:
  - solidify results from juvenile experiment
  - add new juvenile samples to experimental diagram
  - determine which samples to process for molecular analysis
discussed Sam’s upcoming Spring plans