Equipment brought to hatchery:
- HgCl2 (stored in safety cabinet
- 100um silos (15 total)
Equipment returned from hatchery:
- Sam’s N2 dewers with samples
- stored in lab here:
- -80 inventory list
- slack post
- Mesh tubes for outplanting (56 total)
Debrief with Sam:
- Extra spray bar plumbing under sink in dry lab
- repirometry:
- calibration to stickers, factory calibration is better than recalibrating. All read around 200umol/L at start of trial
- ziploc baggie has all the vials
- SDR plate needs to be secured to rotator for next trial (tape, etc.)
FFAR Call 11-12:30:
- Manuscript for broodstock reproductive development
- histology data
- hemolymph over time
- brainstorm what we can do with this
- tag-seq?
- targeted proteomics
- metabolomics
- maternal RNA? Hollie’s preliminary results showed an effect in maternal RNA (56%)
- review Clare’s paper, sperm WGBS
- analyze preliminary WGBS data
- brainstorm what we can do with this
- for next meeting have:
- Timeline for what needs to be done
- results of histology
- results of WGBS
- Manuscript for transgenerational plasticity
- add strip spawn protocol
- add larval conical split out + survival info
Experimental set-up
-
look at Sam’s paper to determine timepoint for measuring acute stress
- played around with setup
- got a conical 2 going with variable low pH treatment and conical 3 going with ambient