Here is a summary of new ideas for the ‘juvenile resistance to stress’ experiment following a discussion with Steven and Brent on Wed. Aug. 7:
I. chronic pH stress (2 months)
- amb H2O w/ elev. hist.
- amb H2O w/ amb. hist.
- var low pH H2O with elev. hist.
- var low pH H2O with amb. hist.
- something like this:
II. acute stress (6 hrs, 24 hours, 48 hours?)
- freeze samples for metabolite(omics?) and epigenetic analysis
- respirometry?
- Possible acute stressors:
- temp
- salinity
- temp + salinity
- lagoon water
- desiccation
Plan for acute stress:
-
Test one stress at a time:
-
Test multiple stressors simultaneously:
- I’m not sure how realistic respirometry would be for this
III. Measurements to make outside of stress trials
- pre-chronic stress baseline measurement
- freeze samples for metabolite(omics?) and epigenetic analysis
- respirometry?
- pre-acute stress measurement after 1 week chronic pH stress?
- freeze samples
- respirometry?
Proposed timeline:
References:
-
Brood exposure and multistress exposure effect on Oyster larvae parker et al.
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(Multigenerational exposure to ocean warming) https://jeb.biologists.org/content/220/4/551
Summary of fall 2018-Spring 2019 samples
I also went through all the samples taken fall 2018-Spring 2019 and came up with these schematics to better understand the samples we have for possible analyses:
-
Broodstock conditioning with constant low pH
-
Broodstock conditioning with variable low pH
Need to discuss all this with the FFAR group on Thur Aug 15