SHELLY A. TRIGG
University of Washington
School of Aquatic and Fishery Sciences, Seattle, WA
(339)-368-0029 ·
strigg@uw.edu · ORCID ID: 0000-0001-6904-4149
PERSONAL
SUMMARY
I am a Systems Biologist with 8+ years of experience in
'omics' technology development and application. My main interests are in research
and public education outreach on how organisms’ molecular networks respond to
human-influenced environmental change. I am currently a Postdoctoral Research
Associate in the lab of Steven Roberts in the School of Aquatic and Fishery
Sciences at the University of Washington researching how ocean acidification
impacts the physiology and epigenetic variation in bivalves.
EDUCATION
Ph.D. in Biology, University of
California San Diego…………………………......................2018
B.S. in Biochemistry Magna
Cum Laude, Simmons College, Boston, MA…..………............2010
RESEARCH SKILLS
Lab Biology
and Automation
- High throughput sequencing library
preparation
- recombinant DNA techniques and
construct design
- PCR, qPCR, and oligo design
- nucleic acid and protein
purification
- immuno-blotting and -precipitation
- yeast and bacteria cell culture
- electroporation and chemical cell
transformation
- Tecan liquid handling robots and
plate readers
Bioinformatics and Computing
- High throughput DNA sequencing data analysis
- metabolomics data analysis
- meta-analysis and data integration
- network analysis using Cytoscape
- R scripting and statistical analysis
- UNIX/Linux shell scripting
- Some Python
- Microsoft Office applications
Training and
Coursework
- Mass spectrometry
data processing and statistics course, UC Davis
(2018)
- Visiting Scientist
Network Analysis Training Program, Broad Institute (2016)
- NSF Graduate Data
Science Workshop, University of Washington (2015)
- Graduate
Biostatistics, UC San Diego (2013)
- Tecan EVO Training
Course (2012)
RESEARCH EXPERIENCE
University
of Washington School of Aquatic and Fishery Sciences, Roberts Lab, Seattle, WA
Postdoctoral
Research Associate…...………………......................................Sept
2018 - Present
Advisor:
Steven Roberts Ph.D.
· Lead
research efforts on how environmental change, like ocean acidification, impacts
the physiology and epigenetic variation in marine invertebrates, particularly
bivalves
NOAA Northwest Fisheries Science Center, Conservation
Biology/Genetics Division, Seattle, WA
Volunteer…...……………………..………………......………………….......Jun
2018 - Present
NSF
Graduate Research Internship Program Fellow…...………………......Jun
2017 - May 2018
Project title: Understanding the physiological and genetic
impacts of ocean acidification on marine invertebrates
Advisors:
Paul McElhany Ph.D. and Krista Nichols Ph.D.
· Performed quality control,
statistical, and physiological pathway analyses of metabolomics datasets from
juvenile Dungeness crab exposed to lab simulated ocean acidification conditions
· Carried out time-course high
throughput ocean acidification exposure experiments on Dungeness crab larvae
· Prepared whole genome sequencing
libraries for the Dungeness crab genome project
· Did field collection and laboratory
rearing of Dungeness crab larvae and adults
Salk Institute for Biological Studies, Ecker Lab Genomic
Analysis Section, La Jolla, CA
NSF
Graduate Research Fellow…………………………………….………...….........2014 -
2018
Graduate
Student Researcher…………………………………….………….…….......2013 - 2018
Ph.D. dissertation: High-resolution
molecular networks from novel 'omics' approaches elucidate survival strategies
in organisms from land to sea
Advisor: Joseph Ecker, Ph.D.
· Developed a next-generation DNA
sequencing assay (CrY2H-seq) for high throughput protein-protein interaction
screening and coded an analysis pipeline to identify and quantify interactions
from large sequence datasets
· Generated a comprehensive
protein-protein interactome map for Arabidopsis
by creating high complexity clone libraries and screening libraries with
CrY2H-seq
· Validated protein interaction
networks found with CrY2H-seq by
performing experimental and computational quality control, in-depth network
analysis, data integration, and functional annotation of protein
Research
Assistant II…………………………………….………...…..........................2012
- 2013
Research
Assistant I…………………………………….………...…............................2011
- 2012
Dana Farber Cancer Institute, Center for Cancer Systems
Biology, Vidal Lab, Boston, MA
Research
Technician I…………………………………….………...…........................2010
- 2011
· Generated
comprehensive human protein-protein interactome using high throughput yeast two-hybrid
screening, protein complementation assay, and well-nucleic-acid-programmable-protein-array
(wNAPPA) assay
·
Generated interactomes using high throughput yeast two-hybrid
screening of autism genes and human isoforms against a human ORFeome
collection
Research
Trainee…………………………………….………...…..................Jan 2010 - Aug 2010
· Constructed
arrayed collections of Kaposi’s Sarcoma and Epstein-Barr viral ORFs by high
throughput gateway cloning for yeast two-hybrid screening against a human
ORFeome to define potential cancer driver and passenger proteins
Simmons College, Department of Chemistry, Roecklein-Canfield
Lab, Boston, MA
Undergraduate
Researcher…………………………………….………...…................2009
- 2010
·
B.S.
Honors Thesis: The effectiveness of the GST fusion system for isolating
Epstein-Barr viral thymidine kinase, a potential target for antiviral antitumor
therapies
PUBLICATIONS
AND MANUSCRIPTS
Shelly A. Trigg,
M Maher, D Perez, DS Busch, P McElhany, and KM Nichols. Uncovering
mechanisms of global ocean change effects on Dungeness
crab (Cancer magister) through
metabolomics analysis. (In preparation)
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R
O’Malley, SC Huang, ZZ Zhang, M Galli, and JR Ecker (2017) CrY2H-seq: a massively multiplexed assay for
deep-coverage interactome mapping. Nat
Methods. 14(8):819-825.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R
O’Malley, SC Huang, ZZ Zhang, M Galli, and JR Ecker (2017) CrY2H-seq interacome screening. Protoc. Exch. doi:10.1038/protex.2017.058.
X Yang, [15 others], Shelly A. Trigg, [20
others], and M Vidal. (2016) Widespread expansion of protein interaction
capabilities by alternative splicing. Cell. 164(4):805-817.
T Rolland, [53 others], Shelly A. Trigg,
[14 others], and M Vidal. (2014) A proteome-scale map of the human interactome
network. Cell. 159(5):1212-1226.
R Corominas, [8 others], Shelly A. Trigg,
[18 others], M Vidal, and LM Iakoucheva. (2014) Protein interaction network of
alternatively spliced isoforms from brain links genetic risk factors for
autism. Nat commun. 5:3650.
O Rozenblatt-Rosen, [36 others], Shelly
Wanamaker, [13 others], and M Vidal. (2012) Interpreting cancer genomes
using systematic host network perturbations by tumour virus proteins. Nature. 487(7408):491-495.
FIRST AUTHOR CONFERENCE
PRESENTATIONS
Shelly A. Trigg,
P McElhany, M Maher, D Perez, DS Busch, and KM Nichols. (Mar 2018) Uncovering
mechanisms of global ocean change effects on Dungeness crab through
metabolomics analysis. NOAA NWFSC 6th Science Symposium, Seattle, WA,
oral.
Shelly A. Trigg,
M Maher, D Perez, DS Busch, P McElhany, and KM Nichols. (Feb 2018) Uncovering
mechanisms of global ocean change effects on Dungeness crab through
metabolomics analysis. Ocean Sciences Meeting, Portland, OR, poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney,
R O’Malley,
CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2016) ProCREate: A New Assay
for Generation of Large-Scale Protein-Protein Interaction Maps, HHMI Scientific
Meeting, Janelia Research Campus, Ashburn, VA, poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2016) ProCREate: A New Assay
for Generation of Large-Scale Protein-Protein Interaction Maps, UCSD Biology
annual retreat, Lake Arrowhead, CA, poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, ZZ Zhang, M Galli, and JR Ecker (Mar 2016) Next-Generation Protein
Interactomes for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for
Bioenergy Meeting, Tyson’s Corner, VA,
poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, ZZ Zhang, M Galli, and JR Ecker (October 2015) The ProCREate
interactome mapping technique reveals crosstalk and directionality between
Arabidopsis regulatory networks, Salk Science at the Seaside, La Jolla, CA,
poster and mini presentation. People’s
Choice Award for best mini presentation.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2015) ProCREate: A New Assay
for Generation of Large-Scale Protein-Protein Interaction Maps, UCSD Biology
annual retreat, Lake Arrowhead, CA, poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, M Galli, and JR Ecker (Aug 2015) Finding biological relevance in
large-scale protein network studies, NSF Data Science Workshop, Seattle, WA,
poster.
Shelly A. Trigg,
RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J
Feeney, R O’Malley,
CS Huang, M Galli, and JR Ecker (Feb 2015) Next-Generation Protein Interactomes
for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for
Bioenergy Meeting, Tyson’s Corner, VA,
poster.
Shelly A. Trigg,
A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R O’Malley, M Galli, and
JR Ecker (Feb 2014) Next-Generation Protein Interactomes for Plant Systems
Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Arlington, VA,
poster.
Shelly A. Trigg,
J Feeney, JR Nery, A Bartlett, R Castanon, A Goubil, R O’Malley, M Galli, and
JR Ecker (Feb 2013) Next-Generation Protein Interactomes for Plant Systems
Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Bethesda, MD,
poster.
ACADEMIC
SERVICE
Teacher training
coursework
Evidence-Based
Teaching and Learning in Biological Sciences, UC San Diego Winter 2017
Introduction to
College Biology Education, UC San Diego Spring 2016
Teaching experience
Head Instructional
Assistant
Regulation of Eukaryotic Gene
Expression, UC San Diego Winter 2017
Instructional
Assistant
Genomics Research
Initiative Laboratory, UC San Diego Spring 2016
Biochemical Techniques Laboratory, UC
San Diego Fall
2014
Graduate Student
Instructor
Quantitative Biology graduate seminar,
UC San Diego
2015 - 2016
Undergraduate Teaching
Assistant
Biochemistry II,
Simmons College Spring
2010
Organic Chemistry I,
Simmons College Spring
2009
Organic Chemistry II,
Simmons College Fall 2008
General Chemistry,
Simmons College
Summer 2008
Mentoring Experience
NOAA Hollings Scholar
undergraduate student Akofa Kekeh
Summer
2017
Biology Department graduate
peer mentor committee chair 2015 - 2016
Graduate peer mentor
of graduate student Eileen Gonzales 2015
- 2016
Graduate peer mentor
of graduate
student Natasha Slepak
2014
- 2015
EDUCATION
OUTREACH
National Marine Sanctuaries ocean acidification multimedia toolkit
development 2017 - 2018
Salk Institute Education Outreach plant biology video media
development 2016
Salk Institute AAAS New Frontiers in Science Education curriculum
developer 2014 - 2017
UC San Diego Undergraduate Biology Showcase poster judge 2015 - 2018
Reuben H. Fleet Science Center #2Scientists program 2014 - 2016
Association for Women in Science (AWIS) San Diego outreach committee
member 2012 - 2016
Expanding Your Horizons San Diego AWIS presenter 2013 - 2015
AWIS San Diego undergraduate career panel coordinator 2014
Albert Einstein Academy Family Science Night presenter 2014
ACS Chemistry Expo AWIS presenter, San Diego 2013
Greater San Diego Science Festival Expo Day presenter 2013 - 2014
Greater San Diego Science Festival poster judge 2013 - 2014
AWARDS
NSF Graduate Research
Internship 2017 - 2018
Excellence in Teaching
Award, UC San Diego Division of Biological Sciences
2016
Salk Institute Annual Retreat poster presentation People’s Choice Award 2015
NSF Graduate Research
Fellowship 2014 - 2018
Rookie of the Year
Award, Association for Women In Science, San Diego
2013
Allen Douglass Bliss
Award in Chemistry for academic achievement and promise in the field 2010
PROFESSIONAL
MEMBERSHIPS
The Ocean Society
American Association
for the Advancement of Science
Association of Women
in Science
Tri Beta Biological
Honors Society
Sigma Xi Biological
Honors Society